Interesting reads:
Randle Lab
Randle, L., Gilbert, T., Malik, H. and Goldring, C (2023) Establishing a patient-derived model of cholangiocarcinoma using precision cut tumour slices (PCTS). Abstract p24.BTS Annual Meeting, April 2023.
Chidlow, S.J., Randle, L.E. and Kelly, R.A (2022) Predicting physiologically-relevant oxygen concentrations in precision-cut liver slices using mathematical modelling. PLOS One, (accepted).
This paper describes tissue oxygenation as a function of slice size, diameter and position in well.
Gilbert, T., Randle, L., Diaz-Neito, R., Jones, R., Fenwick, S., Goldring, C and Malik, H. (2022). Developing a patient-derived model of cholangiocarcinoma using Precision Cut Tissue Slices (PCTS). AUGIS 2022 Meeting, Aberdeen.
Abstract available here: Gilbert AUGIS 2022
Gilbert, T.M., Randle, L., Quinn, M., McGreevy, O., O’Leary, L., Young, R., Diaz-Neito, R., Jones, R.P., Greenhalf, B., Goldring, C., Fenwick, S. Malik, H. and Palmer, D. Molecular biology of cholangiocarcinoma and its implications for targeted therapy in patient management. EJSO. Article in press https://doi.org/10.1016/j.ejso.2024.108352 *Joint first author
McGreevy, O., Bosakhar, M. Gilbert, T., Quinn, M., Fenwick, S. Malik, H., Goldring, C., and Randle, L. 2024. The importance of preclinical models in cholangiocarcinoma. EJSO, Article in press 108304. https://doi.org/10.1016/j.ejso.2024.108304
CCA papers
Banales, J.M., Marin, J.J.G., Lamarca, A., Rodrigues, P.M., Khan, S.A., Roberts, L.R., Cardinale, V., Carpino, G., Andersen, J.B., Braconi, C., Calvisi, D.F., Perugorria, M.J., Fabris, L., Boulter, L., Macias, R.I.R., Gaudio, E., Alvaro, D., Gradilone, S.A., Strazzabosco, M., Marzioni, M., Coulouarn, C., Fouassier, L., Raggi, C., Invernizzi, P., Mertens, J.C., Moncsek, A., Rizvi, S., Heimbach, J., Koerkamp, B.G., Bruix, J., Forner, A., Bridgewater, J., Valle, J.W. and Gores, G.J. (2020) Cholangiocarcinoma 2020: the next horizon in mechanisms and management. Nature Reviews Gastroenterology & Hepatology, 17 (9), 557-588.
doi: 10.1038/s41575-020-0310-z
The 2020 consensus statement which sets out the CCA field to date and areas that need to be addressed.
Brindley, P.J., Bachini, M., Ilyas, S.I., Khan, S.A., Loukas, A., Sirica, A.E., Teh, B.T., Wongkham, S. and Gores, G.J. (2021) Cholangiocarcinoma. Nature Reviews Disease Primers, 7 (1), 65. This paper reviews cholangiocarcinoma, covering its epidemiology, molecular mechanisms, diagnosis, and treatment strategies.
Chen, Z., Guo, P., Xie, X., Yu, H., Wang, Y. and Chen, G. (2019) The role of tumour microenvironment: a new vision for cholangiocarcinoma. Journal of Cellular and Molecular Medicine, 23 (1), 59-69. This paper discusses the role of the tumor microenvironment in cholangiocarcinoma development and progression.
Greten, T.F., Schwabe, R., Bardeesy, N. et al. Immunology and immunotherapy of cholangiocarcinoma. Nat Rev Gastroenterol Hepatol 20, 349–365 (2023). https://doi.org/10.1038/s41575-022-00741-4
This paper examines cholangiocarcinoma's immunology and recent advances in immunotherapy.
Ilyas, S.I., Khan, S., Hallemeier, C. et al. Cholangiocarcinoma — evolving concepts and therapeutic strategies. Nat Rev Clin Oncol 15, 95–111 (2018). https://doi.org/10.1038/nrclinonc.2017.157 This paper reviews evolving therapeutic strategies in the treatment of cholangiocarcinoma.
Fabris L, Perugorria MJ, Mertens J, et al. The tumour microenvironment and immune milieu of cholangiocarcinoma. Liver Int. 2019; 39(Suppl. 1): 63–78. https://doi.org/10.1111/liv.14098 This paper examines the tumour microenvironment and immune landscape of cholangiocarcinoma.
Valle, J.W., Kelley, R.K., Nervi, B., Oh, D-Y. and Zhu, A.X. (2021) Biliary tract cancer. The Lancet, 397 (10272), 428-444. https://doi.org/10.1016/S0140-6736(21)00153-7. This paper provides an overview of biliary tract cancer, including its epidemiology, diagnosis, and treatment options.
Skouteris, N., Papageorgiou, G., Fioretzaki, R., Schizas, D., Kykalos, S., Tolia, M., & Charalampakis, N. (2023). Immune Checkpoint Inhibitors and Combinations with Other Agents in Cholangiocarcinoma. Immunotherapy, 15(7), 487–502. https://doi.org/10.2217/imt-2022-0225 This paper reviews the use of immune checkpoint inhibitors and their combinations with other therapies in the treatment of cholangiocarcinoma.
Conci S, Catalano G, Roman D, Zecchetto C, Lucin E, De Bellis M, Tripepi M, Guglielmi A, Milella M, Ruzzenente A. Current Role and Future Perspectives of Immunotherapy and Circulating Factors in Treatment of Biliary Tract Cancers. Int J Med Sci 2023; 20(7):858-869. doi:10.7150/ijms.82008. https://www.medsci.org/v20p0858.htm This paper discusses the current and future roles of immunotherapy and circulating factors in the treatment of biliary tract cancers.
Manthopoulou E, Ramai D, Dhar J, Samanta J, Ioannou A, Lusina E, Sacco R, Facciorusso A. Cholangiocarcinoma in the Era of Immunotherapy. Vaccines. 2023; 11(6):1062. https://doi.org/10.3390/vaccines11061062 This paper explores the impact and potential of immunotherapy in the treatment of cholangiocarcinoma.
Du, J., Lv, X., Zhang, Z., Huang, Z. and Zhang, E. Revisiting targeted therapy and immunotherapy for advanced cholangiocarcinoma. Frontiers in Immunology. 2023; 14. https://doi.org/110.3389/fimmu.2023.1142690 This paper reviews advancements in targeted therapy and immunotherapy for advanced cholangiocarcinoma.
Tissue Slice Model (Liver)
Kenerson, H.L., Sullivan, K.M., Seo, Y.D., Stadeli, K.M., Ussakli, C., Yan, X., Lausted, C., Pillarisetty, V.G., Park, J.O. and Riehle, K.J. (2020) Tumor slice culture as a biologic surrogate of human cancer. Annals of Translational Medicine, 8 (4).
https://dx.doi.org/10.21037/atm.2019.12.88
Dewyse, L., Reynaert, H. and van Grunsven, L.A. (2021) Best practices and progress in precision-cut liver slice cultures. International journal of molecular sciences, 22 (13), 7137
Doornebal, E. (2021) Human precision-cut tissue slices as personalised immunocompetent ex vivo model for primary and secondary liver cancer thesis, King’s College London.
Olinga, P. and Schuppan, D. (2013) Precision-cut liver slices: a tool to model the liver ex vivo. J Hepatol, 58 (6), 1252-1253.
Review article
Paish, H.L., Reed, L.H., Brown, H., Bryan, M.C., Govaere, O., Leslie, J., Barksby, B.S., Garcia Macia, M., Watson, A. and Xu, X. (2019) A bioreactor technology for modeling fibrosis in human and rodent precision‐cut liver slices. Hepatology, 70 (4), 1377-1391.
This paper discusses the importance of flow and inserts to model fibrosis.
Palma, E., Doornebal, E.J. and Chokshi, S. (2019) Precision-cut liver slices: a versatile tool to advance liver research. Hepatology international, 13 (1), 51-57
This review article demonstrates novel uses of the PCLS model.
Smith, P.F., Gandolfi, A.J., Krumdieck, C.L., Putnam, C.W., Zukoski III, C., Davis, W.M. and Brendel, K. (1985) Dynamic organ culture of precision liver slices for in vitro toxicology. Life sciences, 36 (14), 1367-1375.
The original protocol describing the preparation of tissue slices.
Wu, X., Roberto, J.B., Knupp, A., Kenerson, H.L., Truong, C.D., Yuen, S.Y., Brempelis, K.J., Tuefferd, M., Chen, A. and Horton, H. (2018) Precision-cut human liver slice cultures as an immunological platform. Journal of immunological methods, 455, 71-79.
https://doi.org/10.1016/j.jim.2018.01.012
Li M, Larsen FT, van den Heuvel MC, Gier K, Gorter AR, Oosterhuis D, Bijzet J, de Meijer VE, Ravnskjaer K, Nagelkerke A, et al. Metabolic Dysfunction-Associated Steatotic Liver Disease in a Dish: Human Precision-Cut Liver Slices as a Platform for Drug Screening and Interventions. Nutrients. 2024; 16(5):626. https://doi.org/10.3390/nu16050626
Fridrichs, J., Hamel, W., Kelder, E., van den Hoed, M., van den Heuvel, C., Hulscher, J.B.F. and Olinga, P. Human precision-cut cystic duct and gallbladder slices: a novel method for studying cholangiopathies. Frontiers in Pediatrics. 2023; 11. https://doi.org/10.3389/fped.2023.1058319
Zhang, Y., Wang, Z., Jing, H., Zhang, H., Yan, H., Fan, J., & Zhai, B. (2022). A pre‑clinical model combining cryopreservation technique with precision‑cut slice culture method to assess the in vitro drug response of hepatocellular carcinoma. International Journal of Molecular Medicine, 49, 51. https://doi.org/10.3892/ijmm.2022.5107
Vega JMH, Hong HJ, Loutherback K, Stybayeva G, Revzin A. A Microfluidic Device for Long-Term Maintenance of Organotypic Liver Cultures. Adv Mater Technol. 2023 Jan 24;8(2):2201121. https://doi.org/10.1002/admt.202201121
Wu, X., Roberto, A., Knupp, A.L. et al. (2022). Response of Human Liver Tissue to Innate Immune Stimuli. Frontiers in Immunology, 13. https://doi.org/10.3389/fimmu.2022.811551
Gore, E., Bigaeva, A., Oldenburger, Y. et al. (2019). PI3K inhibition reduces murine and human liver fibrogenesis in precision-cut liver slices. Biochemical Pharmacology, 169. https://doi.org/10.1016/j.bcp.2019.113633
Tumour Slice Model for different cancers (PDAC etc.)
Misra, S., Moro, C.F., Del Chiaro, M., Pouso, S., Sebestyén, A., Löhr, M., Björnstedt, M. and Verbeke, C.S. (2019) Ex vivo organotypic culture system of precision-cut slices of human pancreatic ductal adenocarcinoma. Scientific reports, 9 (1), 1-16.
DOI: 10.1038/s41598-019-38603-w
Sivakumar, R., et al., Organotypic tumor slice cultures provide a versatile platform for immuno-oncology and drug discovery, Oncoimmunology, 2019. 8(12): p. e1670019.
doi: 10.1080/2162402X.2019.1670019
Jiang, X., et al., Long-lived pancreatic ductal adenocarcinoma slice cultures enable precise study of the immune microenvironment, Oncoimmunology, 2017. 6(7): p. e1333210.
DOI: 10.1080/2162402X.2017.1333210
Kayla Sompel, Alex J. Smith, Caroline Hauer, Alamelu P. Elango, Eric T. Clamby, Robert L. Keith, Meredith A. Tennis; Precision Cut Lung Slices as a Preclinical Model for Non–Small Cell Lung Cancer Chemoprevention. Cancer Prev Res (Phila) 1 May 2023; 16 (5): 247–258. https://doi.org/10.1158/1940-6207.CAPR-23-0004
Ghaderi, M., Fernández Moro, C., Pouso Elduayen, S. et al. Genome-wide transcriptome profiling of ex-vivo precision-cut slices from human pancreatic ductal adenocarcinoma. Sci Rep 10, 9070 (2020). https://doi.org/10.1038/s41598-020-65911-3
Hoeffner, C., Worek, F., Horn, G., & Amend, N. (2024). Optimization of long-term incubation of precision-cut kidney slices. Toxicology Mechanisms and Methods, 1–8. https://doi.org/10.1080/15376516.2024.2382797