Setting up slicer
Preparing 5mm cores
Embedding tumour tissue in agarose
Cutting slices
Transferring onto organotypic inserts - Coming soon
Setting up incubation plate (Left normal liver / right tumour)
Collecting slices at timepoint - Coming soon
MTS
Assessing bioenergetics using the Seahorse platform - Coming soon
IHC - Coming soon
Protocol papers
de Graaf, I.A., Olinga, P., de Jager, M.H., Merema, M.T., de Kanter, R., van de Kerkhof, E.G. and Groothuis, G.M. (2010) Preparation and incubation of precision-cut liver and intestinal slices for application in drug metabolism and toxicity studies. nature protocols, 5 (9), 1541.
PMID: 20725069 DOI: 10.1038/nprot.2010.111
This protocol began the renaissance of using tissue slices. Much of the the work to date has used this protocol combining a simple Williams E Media under heyperoxic conditions.
Kenerson, H.L., Sullivan, K.M., Labadie, K.P., Pillarisetty, V.G. and Yeung, R.S. (2021) Protocol for tissue slice cultures from human solid tumours to study therapeutic response. STAR protocols, 2 (2), 100574.
PMID: 34142099 DOI: 10.1016/j.xpro.2021.100574
The protocol our lab uses to prepare hepatobiliary tumour slices under normoxic conditions using organotypic inserts.